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对补充钼的绵羊的肝脏、肾脏、血浆和红细胞中的可溶性钼结合蛋白进行分级分离。

Fractionation of soluble molybdenum-binding proteins from liver, kidney, plasma and erythrocytes from sheep supplemented with molybdenum.

作者信息

Norheim G, Søli N E, Frøslie A, Mjør-Grimsrud M

出版信息

Acta Vet Scand. 1980;21(3):428-37. doi: 10.1186/BF03546875.

DOI:10.1186/BF03546875
PMID:7446324
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8317730/
Abstract

Five ewes were given a daily dose of 1.4 mg Mo/kg body weight as ammonium molybdate for 21 to 55 days. Plasma molybdenum concentrations increased considerably following the dosage, but no systematic changes were seen in the plasma copper concentrations. About 80 of the molybdenum present in blood was found in plasma. With gel filtration it was shown that practically all of the molybdenum present in plasma and red cell haemolyzates was found in a fraction with molecular weight less than 1,500. Soluble proteins from liver and kidney cortex were separated into one high molecular weight fraction with molecular weight above 200,000 and a non-protein fraction with molecular weight less than 1,500. Significant correlations were found between the amounts of molybdenum present in the high molecular weight fraction and the molybdenum concentrations in liver and kidney.

摘要

给5只母羊每日按1.4毫克钼/千克体重的剂量投喂钼酸铵，持续21至55天。给药后血浆钼浓度显著升高，但血浆铜浓度未见系统性变化。血液中约80%的钼存在于血浆中。通过凝胶过滤表明，血浆和红细胞溶血产物中的几乎所有钼都存在于分子量小于1500的组分中。肝脏和肾皮质的可溶性蛋白质被分离为一个分子量高于200,000的高分子量组分和一个分子量小于1500的非蛋白质组分。在高分子量组分中的钼含量与肝脏和肾脏中的钼浓度之间发现了显著相关性。

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Fractionation of soluble molybdenum-binding proteins from liver, kidney, plasma and erythrocytes from sheep supplemented with molybdenum.对补充钼的绵羊的肝脏、肾脏、血浆和红细胞中的可溶性钼结合蛋白进行分级分离。
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本文引用的文献

1
Effect of intake of copper, molybdenum and sulphate on copper metabolism in the sheep. 3. Studies with radioactive copper in male castrated sheep.铜、钼和硫酸盐摄入量对绵羊铜代谢的影响。3. 雄性去势绵羊放射性铜的研究。
J Comp Pathol. 1968 Oct;78(4):449-61. doi: 10.1016/0021-9975(68)90044-3.
2
Effect of intake of copper, molybdenum and sulphate on copper metabolism in sheep. I. Clinical condition and distribution of copper in blood of the pregnant ewe.铜、钼和硫酸盐摄入量对绵羊铜代谢的影响。I. 妊娠母羊的临床状况及血液中铜的分布
J Comp Pathol. 1968 Jul;78(3):351-62. doi: 10.1016/0021-9975(68)90013-3.
3
Effect of dietary molybdenum and sulfate upon urinary excretion of copper in sheep.日粮钼和硫酸盐对绵羊尿铜排泄的影响。
J Nutr. 1970 Dec;100(12):1399-405. doi: 10.1093/jn/100.12.1399.
4
The role of organic sulphur in the copper-molybdenum-S interrelationship in ruminant nutrition.有机硫在反刍动物营养中铜 - 钼 - 硫相互关系中的作用。
Br J Nutr. 1975 Nov;34(3):411-20. doi: 10.1017/s0007114575000475.
5
Determination of protein-bound trace elements in biological material by gel filtration and neutron activation analysis.
Anal Chem. 1975 Aug;47(9):1688-90. doi: 10.1021/ac60359a063.
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Effect of dietary Mo on Cu metabolism. Evidence for the involvement of Mo in abnormal binding of Cu to plasma proteins.
Clin Chim Acta. 1975 Jul 9;62(1):55-63. doi: 10.1016/0009-8981(75)90279-x.
7
Copper: molybdenum interaction. Effect of dietary molybdenum on the binding of copper to plasma proteins in sheep.铜：钼的相互作用。日粮钼对绵羊血浆蛋白结合铜的影响。
J Comp Pathol. 1975 Apr;85(2):299-305. doi: 10.1016/0021-9975(75)90072-9.
8
The concentrations of molybdenum and zinc in liver in relation to copper accumulation in normal and copper poisoned sheep.正常及铜中毒绵羊肝脏中钼和锌的浓度与铜蓄积的关系
Acta Vet Scand. 1976;17(3):307-15. doi: 10.1186/BF03547911.
9
Distribution of some protein-bound trace elements among soluble protein fractions from human liver.人体肝脏可溶性蛋白质组分中某些蛋白质结合微量元素的分布
Acta Pharmacol Toxicol (Copenh). 1976 Feb;38(2):137-44. doi: 10.1111/j.1600-0773.1976.tb03106.x.
10
The influence of dietary sulphur, molybdate and copper on the absorption, excretion and plasma fraction levels of 99Mo in sheep.日粮中的硫、钼酸盐和铜对绵羊体内⁹⁹Mo吸收、排泄及血浆组分水平的影响。
Ann Rech Vet. 1978;9(3):577-86.

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