Yang C C, Chang C C, Lin M F, Lee H Y, Chuang L Y
Int J Pept Protein Res. 1978 Nov;12(5):303-10. doi: 10.1111/j.1399-3011.1978.tb02901.x.
The heterogeneity of precipitating and non-precipitating antibodies to cobrotoxin was demonstrated by their elution pattern on cobrotoxin-Sepharose and chromatography on DEAE-cellulose column. Gel filtration patterns on a Sepharose 6B column revealed that the soluble complexes formed from non-precipitating antibody and HNB-cobrotoxin at a different molar ratio all emerged in the void volume, indicating that the molecular weight of the soluble complex is around 4,000,000 or larger. Unreacted free non-precipitating antibody coincided with the peak of IgG and was proved to be free from HNB-cobrotoxin. The molar ratio of antibody to antigen for the soluble complex was found to be 0.79 to 0.97 indicating that 1.58--1.94 molecules of non-precipitating antibody are bound to HNB-cobrotoxin instead of three molecules as in the case of precipitating antibody.
通过眼镜蛇毒素-琼脂糖凝胶上的洗脱模式以及在二乙氨基乙基纤维素柱上的色谱分析，证实了针对眼镜蛇毒素的沉淀抗体和非沉淀抗体的异质性。在琼脂糖6B柱上的凝胶过滤模式显示，由非沉淀抗体和HNB-眼镜蛇毒素以不同摩尔比形成的可溶性复合物均出现在空体积中，这表明可溶性复合物的分子量约为400万或更大。未反应的游离非沉淀抗体与IgG峰一致，并且被证明不含HNB-眼镜蛇毒素。发现可溶性复合物的抗体与抗原的摩尔比为0.79至0.97，这表明1.58 - 1.94个非沉淀抗体分子与HNB-眼镜蛇毒素结合，而不是像沉淀抗体那样三个分子结合。
