Yamamoto M, Chan-Palay V, Palay S L
Anat Embryol (Berl). 1980;159(2):137-49. doi: 10.1007/BF00304974.
In an attempt to define the potential application of neurotransmitter-specific transport as a method of tracing fiber connections, we have examined the uptake and subsequent ortho- and retrograde transport of tritium-labeled serotonin (3H-5HT) in the cerebellum-raphe pallidus system. Injection of various concentrations of 3H-5HT followed by different post-injection survival times revealed different labeling patterns in the injected sites and different patterns of transport. The most striking feature is that nonseroitonin neurons as well as serotonin cells were able to take up and transport the tritium label in both otho- and retrograde fashion. The non-sertonin-specific nature of this uptake and transport is more obvious at higher concentrations of 3H-5HT (more than 9 X 10(-5) M), with longer survival times and following pretreatment with monoamine oxidase inhibitors. At a concentration of 9 X 10(-6) M 3H-5HT, only specific uptake seems to take place as evidenced by label in known serotinin cells and fiber systems; however, it was impossible to detect by autoradiography any ortho- or retrograde transport at this low concentration. Non-specific uptake and transport were observed following injection into the vestibular nuclei and oculomotor complex. This suggests that non-specific uptake and the transport of 3H-5HT or metabolites may also occur in other regions of the central nervous system.
为了确定神经递质特异性转运作为追踪纤维连接方法的潜在应用，我们研究了小脑-中缝苍白球系统中氚标记的5-羟色胺（3H-5HT）的摄取以及随后的顺行和逆行转运。注射不同浓度的3H-5HT并在注射后给予不同的存活时间，结果显示注射部位有不同的标记模式以及不同的转运模式。最显著的特征是，非5-羟色胺能神经元以及5-羟色胺能细胞都能够以顺行和逆行方式摄取和转运氚标记物。在较高浓度的3H-5HT（超过9×10^(-5) M）、更长的存活时间以及用单胺氧化酶抑制剂预处理后，这种摄取和转运的非5-羟色胺特异性性质更为明显。在3H-5HT浓度为9×10^(-6) M时，只有特异性摄取似乎会发生，这可从已知5-羟色胺能细胞和纤维系统中的标记物得到证明；然而，在此低浓度下通过放射自显影无法检测到任何顺行或逆行转运。在前庭核和动眼神经复合体注射后观察到了非特异性摄取和转运。这表明3H-5HT或其代谢产物的非特异性摄取和转运也可能发生在中枢神经系统的其他区域。
