Goyal H O, Hrudka F
Andrologia. 1980 Sep-Oct;12(5):401-4. doi: 10.1111/j.1439-0272.1980.tb01690.x.
The resorptive activity of the efferent ductules was studied in mature and immature bulls using histochemical, electron microscopical, and experimental methods. Resorptive activity was indicated by the uptake of the protein tracer (HRP) and the presence of microvilli, endocytotic apparatus, and alkaline phosphatase activity. The above features were found in all three types of nonciliated cells, but were apparently best developed in vacuolated (type III) cells. The resorptive apparatus was fully developed by 25 weeks, an age which roughly corresponds with the luminization of seminiferous tubules and the onset of spermatogenesis. In mature bulls the resorptive apparatus was markedly affected by androgen deprivation resulting from orchidectomy and was restored by the administration of testosterone, indicating its dependence upon the circulating androgen. Efferentiectomy had little impact, indicating that the luminal androgen does not play a major role in maintaining the resorptive apparatus. The tracer study revealed that the specific granules and vacuoles of type II and III cells, respectively, are not associated with resorptive function. It also showed that the tracer injected into the rete testis took 6 to 24 hours to pass through the efferent ductules and reach the initial segment of the epididymis.
运用组织化学、电子显微镜及实验方法，对成熟和未成熟公牛的输出小管的重吸收活性进行了研究。蛋白质示踪剂（辣根过氧化物酶）的摄取以及微绒毛、内吞装置和碱性磷酸酶活性的存在表明了重吸收活性。上述特征在所有三种类型的无纤毛细胞中均有发现，但在空泡化（III型）细胞中显然最为发达。重吸收装置在25周时发育完全，这个年龄大致与生精小管的管腔形成和精子发生的开始相对应。在成熟公牛中，重吸收装置受到去势导致的雄激素剥夺的显著影响，并通过给予睾酮得以恢复，表明其对循环雄激素的依赖性。输出小管切除术影响不大，表明管腔内雄激素在维持重吸收装置方面不起主要作用。示踪剂研究表明，II型和III型细胞的特定颗粒和空泡分别与重吸收功能无关。研究还表明，注入睾丸网的示踪剂需要6至24小时才能穿过输出小管并到达附睾起始段。
