Kudirka D T, Van't Hof J
Anal Quant Cytol. 1980 Jun;2(2):99-106.
Cells of the mature petal contain two types of nuclei that differ in the degree of chromatin compaction, and it was found that the degree of compaction was a source of error in cytophotometric measurements of Feulgen-stained DNA. The error, which was observed as a deviation from the Beer-Lambert law, resulted in underestimation of total nuclear Feulgen-DNA values. It was shown, however, that in spite of this deviation, segregation of the nuclei into discrete 2c and 4c DNA content classes was still possible because (1) the mean extinction of a nucleus is a function of nuclear area, and (2) nuclei with different amounts of DNA have dissimilar mathematical functions. The cytophotometric results were supported by autoradiography and biochemical measurements. In addition, it was demonstrated that the error was greater for nuclei with the more compacted chromatin and that a large portion of the error was associated with the nuclear periphery.
成熟花瓣的细胞含有两种在染色质压缩程度上存在差异的细胞核，并且发现这种压缩程度是福尔根染色DNA细胞光度测量中误差的一个来源。该误差表现为偏离比尔-朗伯定律，导致总核福尔根-DNA值被低估。然而，结果表明，尽管存在这种偏差，细胞核仍有可能被分离为离散的2c和4c DNA含量类别，原因如下：（1）细胞核的平均消光值是核面积的函数，（2）具有不同DNA含量的细胞核具有不同的数学函数。细胞光度测量结果得到了放射自显影和生化测量的支持。此外，还证明了对于染色质压缩程度更高的细胞核，误差更大，并且大部分误差与核周边有关。
