[Effect of methionine and vitamins B12 and B15 on the content and distribution of proteins, glycogen and lipids in the hepatic lobules of chickens].

The effect of methionine (10 g/kg of food) added in combination with vitamin B12 (25 mkg/kg of food) and vitamin B15 (20 mg/kg of body weight) on the content of glycogen, protein and lipids in the chicken hepatic lobule has been studied on the background of a disbalanced diet. To the disbalanced ration 5% of rancid technical oil was added. The preparations were subjected to photometry in the scanning integrating microscope CIM-1. Comparing with the control, the disbalanced diet decreases the content of glycogen in the liver by 7%, protein--by 27% and increases that of lipids by 32%. Methionine added in combination with vitamins B12 and B15 normalizes the hepatic histochemical indices. Glycogen is firstly accumulated along the lobular periphery and it is this part of glycogen that is mostly subjected to both hepatotrophic and normalizing effects. Lipids also accumulate from the periphery towards the lobular center. The process of normalization of the lipid content is evidently directed from the center towards the periphery. In the central lobular part the protein content is increasing, the process being more pronounced when methionine is added in combination with vitamin B12 than with vitamin B15.