Stevens J, Eisen H J, Stevens Y W, Haubenstock H, Rosenthal R L, Artishevsky A
Cancer Res. 1981 Jan;41(1):134-7.
We have explored the possibility of using antibodies against purified rat liver glucocorticoid receptors to study the immunochemical properties of glucocorticoid receptors from murine and human malignant lymphocytes. For this purpose, purified immune immunoglobulin G was covalently linked to Sepharose CL-4B. We then examined the ability of the affinity gel to recognize cytosolic [3H]triamcinolone acetonide-receptor complexes from the corticoid-sensitive (CS) and -resistant strains of mouse lymphoma P1798, from CS lymphocytes of patients with chronic lymphatic leukemia, and from a CS clone of human leukemic lymphoblasts in tissue culture (CH6). Mouse thymus was used as a source of glucocorticoid receptor from normal CS lymphocytes. Whereas the immunoaffinity column retained 70 to 84% of the 58- to 62-A (Stokes radius) [3H]triamcinolone acetonide-receptor complexes characteristic of the CS mouse and human lymphocytes, it failed to recognize the 27- to 28-A (Stokes radius) glucocorticoid receptor present in corticoid-resistant mouse lymphoma P1798 cells. Therefore, under appropriate experimental conditions, it was possible to demonstrate cross-reactivity between the antiserum against rat liver glucocorticoid receptor and the 58- to 62-A (Stokes radius) glucocorticoid receptor from species as diverse as mouse and humans.
我们探讨了使用抗纯化大鼠肝脏糖皮质激素受体的抗体来研究小鼠和人类恶性淋巴细胞中糖皮质激素受体免疫化学特性的可能性。为此，将纯化的免疫免疫球蛋白G与琼脂糖CL-4B共价连接。然后，我们检测了亲和凝胶识别来自小鼠淋巴瘤P1798的糖皮质激素敏感（CS）和抗性品系、慢性淋巴细胞白血病患者的CS淋巴细胞以及组织培养中的人类白血病淋巴母细胞CS克隆（CH6）的胞质[³H]曲安奈德-受体复合物的能力。小鼠胸腺用作正常CS淋巴细胞糖皮质激素受体的来源。免疫亲和柱保留了CS小鼠和人类淋巴细胞特有的58至62埃（斯托克斯半径）[³H]曲安奈德-受体复合物的70%至84%，但未能识别存在于糖皮质激素抗性小鼠淋巴瘤P1798细胞中的27至28埃（斯托克斯半径）糖皮质激素受体。因此，在适当的实验条件下，有可能证明抗大鼠肝脏糖皮质激素受体抗血清与来自小鼠和人类等不同物种的58至62埃（斯托克斯半径）糖皮质激素受体之间存在交叉反应性。
