Comparison of nuclear proteins of several human tumors and normal cells by two-dimensional gel electrophoresis.

Using isoelectric focusing and sodium dodecyl sulfate two-dimensional gel electrophoresis, 9 M urea-extractable nuclear proteins from four human tumor cells (HeLa, Namalwa, acute myelogenous leukemia, and lymphoma) and four normal human cells (IMR-90, WI-38, liver, and lymphocytes) were compared. Two protein spots, 140/7.7 and 54/6.6, were found in all four tumor cells but not in the four normal cells studied. Two protein spots, 56/6.7 and 56/6.9, were found in all four normal cells but not in any of the tumors studied. None of these proteins was common to those found in the earlier studies on rat tumors (H. Takami et al., Cancer Res., 39: 2096-2105, 1979).