Sisskin E E, Barrett J C
Cancer Res. 1981 Jan;41(1):346-50.
Treatment of initiated hamster skin with 12-O-tetradecanoylphorbol-13-acetate does not promote the formation of papillomas and carcinomas as it does in the classic two-stage epidermal carcinogenesis model in many strains of mice. We have begun to examine the basis of this species specificity to TPA. We have found that, although hamster and mouse epidermis respond to a single exposure of TPA with a comparable degree of hyperplasia, they differ in their response to multiple treatments. In contrast to the reported potentiation of hyperplasia of mouse skin following two or more treatments with TPA, hamster skin is capable of adapting to multiple exposures to the promoter. Weekly TPA treatments of hamster skin result in hyperplasia of 4 to 6 nucleated cell layers after the first treatment, reduced hyperplasia after the second treatment, and no hyperplasia after 4 weeks of treatment. Decreasing the time interval between treatments accelerates the adaptation process. This adaptation phenomenon should be useful in studying the mechanism of TPA action by examining TPA effects in adapted and nonadapted tissues from the same species.
用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯处理起始的仓鼠皮肤，并不会像在许多品系小鼠的经典两阶段表皮致癌模型中那样促进乳头状瘤和癌的形成。我们已经开始研究这种对佛波酯（TPA）的物种特异性的基础。我们发现，尽管仓鼠和小鼠表皮对单次TPA暴露的增生程度相当，但它们对多次处理的反应有所不同。与报道的用TPA进行两次或更多次处理后小鼠皮肤增生增强相反，仓鼠皮肤能够适应多次暴露于启动子。每周用TPA处理仓鼠皮肤，第一次处理后会导致4至6个有核细胞层增生，第二次处理后增生减少，处理4周后不再有增生。缩短处理间隔时间会加速适应过程。这种适应现象通过检查同一物种适应和未适应组织中的TPA效应，对于研究TPA作用机制应该是有用的。
