关于人和某些动物血清及组织鸟嘌呤酶性质的进一步观察。8-氮杂鸟嘌呤存在时的最适pH值和活化能。

Further observations on the properties of serum and tissue guanase from man and some animal species. Optimum pH and activation energy in the presence of 8-azaguanine.

作者信息

Galanti B, Russo M, Nardiello S, Giusti G

出版信息

Enzyme. 1976;21(4):342-8. doi: 10.1159/000458877.

Abstract

The activation energy and the optimum pH of guanine deaminase in man, the rat, guinea pig and mouse were studied using 8-azaguanine as a substrate. The serum guanase in man and in all the animal species studied differs in activation energy from the guanase of the liver. In man, moreover, the serum guanase is also different from the brain and kidney enzyme. In the rat and guinea pig the brain enzyme has thermic activation energy different from the liver and kidney enzyme. The guanase of the serum and tissues of the guinea pig differs from the enzyme of the serum and tissues of man, rat and mouse for optimum pH.

摘要

以8-氮杂鸟嘌呤为底物，研究了人、大鼠、豚鼠和小鼠体内鸟嘌呤脱氨酶的活化能和最适pH值。所研究的所有人及动物物种的血清鸟嘌呤酶，其活化能与肝脏鸟嘌呤酶不同。此外，在人身上，血清鸟嘌呤酶也与脑和肾中的酶不同。在大鼠和豚鼠中，脑酶的热活化能与肝和肾酶不同。豚鼠血清和组织中的鸟嘌呤酶，其最适pH值与人、大鼠和小鼠血清及组织中的酶不同。