静脉注射γ-氨基丁酸（GABA）和脑提取物对大鼠血压的影响。

The effect of intravenous administration of GABA and brain extracts on blood pressure in the rat.

Horvath J S, Baxter C R, Duggin G, Tiller D J

Clin Exp Pharmacol Physiol. 1980 Sep-Oct;7(5):481-5. doi: 10.1111/j.1440-1681.1980.tb00096.x.

Abstract

A rat bioassay was used to study pressor substances in brain extracts. 2. A compound with pressor activity in pentolinium-treated rats was extracted from brain, purified using Dowex, Sephadex electrophoresis and identified as gamma-aminobutyric acid (GABA) by HPLC and mass spectrometry. 3. Intravenous GABA (1-5 microgram) or brain extracts caused a dose dependent decrease in blood pressure in pentobarbitone-anaesthetized rats. 4. After ganglion blockade with pentolinium (2 mg/kg subcutaneously), GABA or brain extracts cause a dose dependent increase in rat blood pressure. 5. This pressor effect is reduced by alpha- and beta-blockers (intravenous phentolamine, 0.5 mg/kg and propranolol, 0.05 mg/kg). 6. It is concluded that GABA causes a fall in blood pressure by a central mechanism and ganglion blockade unmasks a pressor effect.

摘要

采用大鼠生物测定法研究脑提取物中的升压物质。2. 从脑中提取出一种在喷托铵处理的大鼠中具有升压活性的化合物，使用Dowex、Sephadex电泳进行纯化，并通过高效液相色谱法和质谱法鉴定为γ-氨基丁酸（GABA）。3. 静脉注射GABA（1 - 5微克）或脑提取物可使戊巴比妥麻醉的大鼠血压呈剂量依赖性下降。4. 用喷托铵（2毫克/千克皮下注射）进行神经节阻断后，GABA或脑提取物可使大鼠血压呈剂量依赖性升高。5. α和β受体阻滞剂（静脉注射酚妥拉明，0.5毫克/千克和普萘洛尔，0.05毫克/千克）可减弱这种升压作用。6. 得出的结论是，GABA通过中枢机制导致血压下降，神经节阻断则揭示出一种升压作用。