Visser T J, Krieger-Quist L M, Docter R, Hennemann G
J Endocrinol. 1978 Dec;79(3):357-62. doi: 10.1677/joe.0.0790357.
The development of a highly sensitive and specific radioimmunoassay for 3,3'-di-iodothyronine (3,3'-T2) is described. The assay was applied to the measurement of 3,3'-T2 in unextracted human serum and used 8-anilino-1-naphthalene-sulphonic acid to inhibit the binding of 3,3'-T2 to serum transport proteins. The lower limit of detection of the assay was 2 fmol 3,3'-T2 per tube, which corresponded to 10 pmol 3,3'-T2/l serum. The mean concentration of 3,3'-T2 in normal serum was found to be 23 pmol/l, which is considerably lower than most values reported previously. Evidence is presented which suggests that the cross-reactivity of tri-iodothyronine with the antiserum to 3,3'-T2 is an important factor in the measurement of serum concentrations of 3,3'-T2 by radioimmunoassay.
本文描述了一种用于3,3'-二碘甲状腺原氨酸（3,3'-T2）的高灵敏度和特异性放射免疫分析方法的开发。该分析方法应用于未提取的人血清中3,3'-T2的测量，并使用8-苯胺基-1-萘磺酸来抑制3,3'-T2与血清转运蛋白的结合。该分析方法的检测下限为每管2 fmol 3,3'-T2，相当于血清中10 pmol 3,3'-T2 / l。发现正常血清中3,3'-T2的平均浓度为23 pmol / l，这大大低于先前报道的大多数值。有证据表明，三碘甲状腺原氨酸与抗3,3'-T2血清的交叉反应性是通过放射免疫分析测量血清中3,3'-T2浓度的一个重要因素。
