Chuang C Y, Luo S F
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1980 Mar;13(1):75-88.
Immunoelectrophoresis is a combined technique of electrophoresis and double diffusion. The test has been widely used to enumerate and analyze the antigenic components in mixtures, and it is indispensable in the study of monoclonal gammopathy. The interpretation of reaction lines needs basic immunochemical knowledge about immunoglobulin and principles of immunoprecipitation. When properly performed, immunoelectrophoresis will, in most instances, confirm the diagnosis and establish the class and type of M protein. However, the reaction lines should be interpreted cautiously, and the immunoelectrophoretic pattern must be correlated with that of electrophoresis and also with the immunoglobulins levels. The basic immunoelectrophoretic patterns of most frequently encountered monoclonal gammopathies are presented and some of the difficult problems are discussed in this report.
免疫电泳是一种将电泳和双向扩散相结合的技术。该检测已被广泛用于混合抗原成分的计数和分析，在单克隆丙种球蛋白病的研究中不可或缺。反应线的解读需要有关免疫球蛋白的基础免疫化学知识和免疫沉淀原理。如果操作得当，免疫电泳在大多数情况下将确诊并确定M蛋白的类别和类型。然而，对反应线的解读应谨慎，免疫电泳图谱必须与电泳图谱以及免疫球蛋白水平相关联。本报告展示了最常见的单克隆丙种球蛋白病的基本免疫电泳图谱，并讨论了一些难题。
