Lu C H, Tsai M Y
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1980 Jun;13(2):138-42.
Attempts were made to determine the lethality of Formosan snake venoms in mice by various inoculation routes. No matter what inoculation routes (s.c. i.p. i.v.) and which mouse strains (ICR, NIH) were used, statistical analysis revealed that the lethality (LD50) of B. multicinctus venom was the greatest followed by N.n. atra, Tr. gramineus, Tr. mucrosquamatus and Agk. acutus venom. The i.v. inoculation resulted in the highest lethality of haemorrhagic venoms (Tr. gramineus, Tr. mucrosquamatus, Agk. acutus) and the i.p. was the next, while in the case of neurotropic venoms (B. multicinctus, N.n. atra) the results were reversed, moreover, the s.c. was the lowest. The reproducibility of different inoculation routes on determining the lethality of venom was expressed by coefficient of variation (S.D./mean). The i.p. was the best inoculation route for haemorrhagic and N.n. atra venoms and the i.v. was the best for B. multicinctus venom. The results obtained from mouse ICR strain seemed to be more reproducible than that obtained from NIH strain. The best method for determining the neutralizing capacity of B. multicinctus antivenin was the i.p. inoculation in ICR mouse.
尝试通过各种接种途径测定台湾蛇毒对小鼠的致死性。无论使用何种接种途径（皮下、腹腔内、静脉内）以及何种小鼠品系（ICR、NIH），统计分析表明，多环扁尾海蛇毒液的致死性（LD50）最高，其次是眼镜蛇、竹叶青、白唇竹叶青和尖吻蝮毒液。静脉内接种导致出血性毒液（竹叶青、白唇竹叶青、尖吻蝮）的致死性最高，腹腔内接种次之，而对于神经毒性毒液（多环扁尾海蛇、眼镜蛇），结果则相反，此外，皮下接种致死性最低。不同接种途径在测定毒液致死性方面的可重复性用变异系数（标准差/平均值）表示。腹腔内接种是出血性毒液和眼镜蛇毒液的最佳接种途径，静脉内接种是多环扁尾海蛇毒液的最佳途径。从ICR小鼠品系获得的结果似乎比从NIH小鼠品系获得的结果更具可重复性。测定多环扁尾海蛇抗蛇毒血清中和能力的最佳方法是对ICR小鼠进行腹腔内接种。
