Heveran J E, Cox M, Tonchen A, Bergamini J A
J Forensic Sci. 1978 Jul;23(3):470-80.
A latex-agglutination inhibition test was developed and evaluated for the detection of barbiturates, primarily in urine, but it has applicability to serum. The 2-h test was performed with samples free of gross debris in a 37 degree C heat block and the reaction between the barbiturate antibody and the barbiturate-latex, at pH 7.3, was inhibited by urines containing secobarbital, amobarbital, pentobarbital, butabarbital, or phenobarbital. For laboratory standards the test was particularly sensitive to secobarbital (300 microgram/litre) and relatively insensitive to amobarbital (5000 microgram/litre). In clinical specimens some barbiturates were detected at levels as low as 150 microgram/litre. The test was specific, and negative endpoints were frequently noted in as little as 30 min. The effects of variables (antiserum dilution, urine specific gravity, heat block temperature, sample size, and type of barbiturate) on the degree of agglutination were also determined.
已开发并评估了一种乳胶凝集抑制试验，用于检测巴比妥类药物，主要检测尿液中的巴比妥类药物，但也适用于血清。该2小时试验在37℃加热块中对无明显碎屑的样品进行，在pH 7.3条件下，含有司可巴比妥、异戊巴比妥、戊巴比妥、布他比妥或苯巴比妥的尿液可抑制巴比妥类抗体与巴比妥类乳胶之间的反应。对于实验室标准，该试验对司可巴比妥（300微克/升）特别敏感，而异戊巴比妥（5000微克/升）相对不敏感。在临床标本中，一些巴比妥类药物的检测水平低至150微克/升。该试验具有特异性，通常在短短30分钟内即可观察到阴性终点。还确定了变量（抗血清稀释度、尿比重、加热块温度、样本量和巴比妥类药物类型）对凝集程度的影响。
