Meibach R C, Maayani S, Green J P
Eur J Pharmacol. 1980 Oct 31;67(4):371-82. doi: 10.1016/0014-2999(80)90178-8.
Binding of D-[3H]lysergic acid diethylamide (LSD) to rat coronal brain slices and its blockade by 5-hydroxytryptamine (5-HT) had characteristics similar to those of brain homogenates in respect of KD, kinetics and reversibility of binding. Radioautography was done on slices that had been incubated in 6 nM [3H] LSD and on adjacent slices incubated in the same concentration of tritiated LSD plus 10(-5) M of 5-HT. Choroid plexus showed densest labeling of [3H] LSD. In neuropil, dense labeling occurred within parts of the hippocampal formation except for fields CA2 and CA3 which were sparsely labeled. All layers of the cortex except the posterior cingulate gyrus were labeled by LSD. 5-HT blocked labeling of choroid plexus, hippocampal formation, septum, pons, medulla and parts of cortex but only reduced labeling of most other structures. LSD binding sites may relate to some of its pharmacological effects.
D-[3H]麦角酸二乙酰胺（LSD）与大鼠冠状脑片的结合及其被5-羟色胺（5-HT）阻断的情况，在结合的解离常数（KD）、动力学和可逆性方面，其特征与脑匀浆相似。对在6 nM [3H] LSD中孵育的脑片以及在相同浓度的氚化LSD加10^(-5) M 5-HT中孵育的相邻脑片进行放射自显影。脉络丛显示出[3H] LSD的最密集标记。在神经毡中，海马结构的部分区域出现密集标记，但CA2和CA3区标记稀疏。除后扣带回外，皮质的所有层均被LSD标记。5-HT阻断了脉络丛、海马结构、隔区、脑桥、延髓和部分皮质的标记，但仅减少了大多数其他结构的标记。LSD结合位点可能与其某些药理作用有关。
