Christensen G M, Riedel B L
Int J Environ Anal Chem. 1980;8(4):277-82. doi: 10.1080/03067318008071896.
The enzymes cholinesterase and urease were mixed individually with gelatin and immobilized onto the inside surface of glass capillary tubes. After the gelatin-enzyme mixture had dried, water samples containing various enzyme inhibiting test chemicals were pumped through the tubes to enable interaction to proceed between the foreign agents and the enzymes. Substrate for each enzyme was later passed through the tubes and any differences in relative enzyme activity determined. This procedure may be useful for health and environmental studies, particularly in the rapid testing for micro- and nanomolar quantities of enzyme-inhibiting pollutants in natural waters and complex effluents.
将胆碱酯酶和脲酶分别与明胶混合，并固定在玻璃毛细管的内表面。在明胶 - 酶混合物干燥后，将含有各种酶抑制测试化学品的水样泵入管中，以使外来物质与酶之间能够发生相互作用。随后将每种酶的底物通过管子，并测定相对酶活性的任何差异。该程序可能对健康和环境研究有用，特别是在快速检测天然水和复杂废水中微摩尔和纳摩尔量的酶抑制污染物方面。
