聚四氟乙烯线圈在液相色谱柱后光化学反应器中的应用——在药物分析中的应用

Use of PTFE coils in post-column photochemical reactors for liquid chromatography--application to pharmaceuticals.

作者信息

Scholten A H, Welling P L, Brinkman U A, Frei R W

出版信息

J Chromatogr. 1980 Oct 31;199:239-48. doi: 10.1016/s0021-9673(01)91376-7.

Abstract

The advantages and the performance of PTFE reaction coils in photochemical reaction detectors for high-performance liquid chromatography (HPLC) are discussed. The excellent performance of these materials at irradiation wavelengths below 300 nm is based on a diffuse radiation transfer and an internal reflectance (light-tube) effect. Optimal coil designs can be obtained from signal vs. flow-rate curves for a particular application. Lamp performance has to be tested periodically and the reactor should be equipped with an efficient cooling system. Application of the principle to the fluorimetric detection of clobazam and phenothiazines after HPLC separation is discussed. The sensitive detection of these pharmaceuticals and their metabolites in serum with a minimum of sample handling demonstrates the potential of photochemical reaction detectors.

摘要

讨论了聚四氟乙烯反应盘管在高效液相色谱（HPLC）光化学反应检测器中的优点及性能。这些材料在低于300 nm的辐射波长下的优异性能基于漫射辐射传递和内反射（光管）效应。针对特定应用，可从信号与流速曲线获得最佳盘管设计。必须定期测试灯的性能，并且反应器应配备高效的冷却系统。讨论了该原理在HPLC分离后氯巴占和吩噻嗪荧光检测中的应用。以最少的样品处理对血清中的这些药物及其代谢物进行灵敏检测，证明了光化学反应检测器的潜力。