Phillips D M, Shalgi R M
J Exp Zool. 1980 Jul;213(1):1-8. doi: 10.1002/jez.1402130102.
Structural and sperm binding properties of the hamster zona pellucida (ZP) were investigated using the scanning electron microscope (SEM). The internal and external surfaces of the ZP were found to be morphologically dissimilar. The external surface displayed a fenestrated lattice-like appearance, whereas the internal surface showed a regular rough appearance. In order to investigate the physiological characteristics of the ZP, zonae were incubated with the vitro capacitated spermatozoa for five minutes and subsequently prepared from SEM examination. Numerous non-acrosome-reacted spermatozoa and dissociated acrosomes were observed associated with the external surface of the ZP. Spermatozoa or acrosomes were, however, not observed associated with the internal surface of the zona. Acrosome-reacted spermatozoa did not adhere to the ZP. It was concluded that the external and internal surfaces of the zona are morphologically and physiologically dissimilar.
利用扫描电子显微镜（SEM）研究了仓鼠透明带（ZP）的结构和精子结合特性。发现ZP的内表面和外表面在形态上不同。外表面呈现出有孔的格子状外观，而内表面则呈现出规则的粗糙外观。为了研究ZP的生理特性，将透明带与体外获能的精子孵育5分钟，随后制备用于SEM检查。观察到许多未发生顶体反应的精子和游离的顶体与ZP的外表面相关联。然而，未观察到精子或顶体与透明带的内表面相关联。发生顶体反应的精子不粘附于ZP。得出的结论是，透明带的外表面和内表面在形态和生理上是不同的。
