Khulordava K G, Kosaganov Iu N, Lazurkin Iu S
Mol Biol (Mosk). 1978 Nov-Dec;12(6):1390-407.
The rate constants of forward and reverse reactions between methylol derivatives of beta-alanine and deoxycytidine 5'-phosphate, deoxyadenosine 5'phosphate and deoxyguanosine 5'phosphate and the equilibrium constants of these reactions were determined by the spectrophotometric method at 39,5 degrees C and pH 6,95. Besides, the equilibrium constant of the reaction between beta-alanine and formaldehyde was determined. Unlike deoxycytidine and deoxyadenosine 5'-phosphates, interaction of deoxyguanosine 5'phosphate with methylol derivatives is more complicated. A model proposed for the interaction of deoxyguanosine 5'phosphate with methylol derivatives explains the behavior of this nucleotide in the reaction. The kinetic and equilibrium constants of the interaction of methylol derivatives with nucleotides investigated exceed by two or three orders of magnitude the corresponding constants of the interaction of formaldehyde with these nucleotides.
在39.5℃和pH 6.95条件下，采用分光光度法测定了β-丙氨酸的羟甲基衍生物与脱氧胞苷5'-磷酸、脱氧腺苷5'-磷酸和脱氧鸟苷5'-磷酸之间正向和逆向反应的速率常数以及这些反应的平衡常数。此外，还测定了β-丙氨酸与甲醛反应的平衡常数。与脱氧胞苷5'-磷酸和脱氧腺苷5'-磷酸不同，脱氧鸟苷5'-磷酸与羟甲基衍生物的相互作用更为复杂。所提出的脱氧鸟苷5'-磷酸与羟甲基衍生物相互作用的模型解释了该核苷酸在反应中的行为。所研究的羟甲基衍生物与核苷酸相互作用的动力学和平衡常数比甲醛与这些核苷酸相互作用的相应常数高出两到三个数量级。
