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[兔血浆、心肌和心脏脂肪组织中脂蛋白脂肪酶、三酰甘油脂肪酶、单酰甘油脂肪酶和三丁酸甘油酯酶活性的测定]

[Determination of the activities of lipoprotein lipase, triacylglycerolipase, monoacylglycerolipase, and tributyrinase in the plasma, myocardium, and cardiac adipose tissue of rabbits].

作者信息

Efimova L V, Malakhova E A

出版信息

Vopr Med Khim. 1980 Mar-Apr;26(2):270-5.

PMID:7456359
Abstract

Fatty emulsion intralipid, activated by rabbit blood plasma, intralipid, micellar emulsion of monooleine and tributyryne emulsion were used, respectively, as substrates for estimation of asctivities of lipoprotein lipase (EC 3.1.1.34), triacyl glycerolipase (EC 3.1.1.3), monoacyl glycerolipase (EC 3.1.1.23) and tributyrynase (EC 3.1.1.13) by modified Dol's method in rabbit heart tissue, heart fatty tissue and blood plasma. Effect of heparin on extraction of lipolytic enzymes from tissues was studied.

摘要

分别使用经兔血浆激活的脂肪乳剂英脱利匹特、英脱利匹特、单油酸胶束乳剂和三丁酸甘油酯乳剂作为底物,采用改良的多尔氏方法测定兔心脏组织、心脏脂肪组织和血浆中脂蛋白脂肪酶(EC 3.1.1.34)、三酰基甘油酯酶(EC 3.1.1.3)、单酰基甘油酯酶(EC 3.1.1.23)和三丁酸甘油酯酶(EC 3.1.1.13)的活性。研究了肝素对从组织中提取脂解酶的影响。

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