Lewis E J, Schwartz M M, Pauli B U, Sharon Z
Nephron. 1978;22(1-3):91-6. doi: 10.1159/000181427.
The sequential intravenous injections of protamine and heparin into rats results in the deposition of protamine-heparin aggregates along the lamina rara externa of the glomerular basement membrane. Morphologic studies indicate that these foreign aggregates are removed from the glomerular basement membrane by virtue of phagocytic activity by the glomerular visceral epithelial cells. We have studied the disappearance rate of protamine-heparin aggregates from the glomerular basement membrane in animals given a single dose of these compounds and in animals receiving chronic administration. The disappearance rate was measured utilizing a computerized morphometric technique to determine the numerical density of protamine-heparin aggregates per 100 micrometer glomerular basement membrane in sequential biopsy specimens taken from 15 to 600 min post-injection. The disappearance rate described a linear function in both acute and chronic animals, with a half-disappearance time of approximately 120 min. Disappearance of the aggregates appeared to be due to epithelial cell phagocytosis. This model provides a method of studying this function in the intact glomerulus in both normal and disease states.
向大鼠静脉内依次注射鱼精蛋白和肝素，会导致鱼精蛋白 - 肝素聚集体沿肾小球基底膜的外侧板层沉积。形态学研究表明，这些外来聚集体通过肾小球脏层上皮细胞的吞噬活性从肾小球基底膜清除。我们研究了单次给药动物和长期给药动物中鱼精蛋白 - 肝素聚集体从肾小球基底膜的消失速率。利用计算机形态测量技术测量消失速率，以确定在注射后15至600分钟采集的连续活检标本中，每100微米肾小球基底膜中鱼精蛋白 - 肝素聚集体的数量密度。在急性和慢性动物中，消失速率均呈线性函数，半消失时间约为120分钟。聚集体的消失似乎是由于上皮细胞吞噬作用。该模型提供了一种在正常和疾病状态下完整肾小球中研究此功能的方法。
