Fidy J, Gáspár S, Raksányi K
Acta Biochim Biophys Acad Sci Hung. 1980;15(1):65-71.
UV photoproducts were induced in two types of uracil solid phase systems: in evaporated and recrystallized thin layers and in dried aquaeous solution respectively. The nature of the photochemical reactions in the two systems was compared by gradient elution column chromatography. In the case of dried solution two types of cyclobutane dimers were isolated, but in recrystallized thin layers only one type was found. The efficiency of the reaction in evaporated layers was much higher than in those produced by drying. Based on the crystal structure of uracil and previous absorption spectroscopic studies of the photochemistry of thin crystalline layers it was stated, that the single type of photodimers produced in evaporated thin layers is characteristic for the photochemical reaction proceeding in crystal matrix; and its structure was proposed to be identical with that of the cis-syn stereoisomer dimer.
分别在蒸发和重结晶的薄层以及干燥的水溶液中。通过梯度洗脱柱色谱法比较了两种体系中光化学反应的性质。在干燥溶液的情况下，分离出了两种类型的环丁烷二聚体，但在重结晶薄层中仅发现了一种类型。蒸发层中反应的效率远高于干燥产生的层中的反应效率。基于尿嘧啶的晶体结构以及先前对薄晶体层光化学的吸收光谱研究表明，蒸发薄层中产生的单一类型的光二聚体是晶体基质中进行的光化学反应的特征；并且提出其结构与顺式-顺式立体异构体二聚体的结构相同。
