结合粘度增量与调和平均旋转弛豫时间来确定溶液中生物大分子的构象。
The combination of the viscosity increment with the harmonic mean rotational relaxation time for determining the conformation of biological macromolecules in solution.
作者信息
Harding S E
出版信息
Biochem J. 1980 Aug 1;189(2):359-61. doi: 10.1042/bj1890359.
Abstract
A new hydrodynamic shape function, lambda, is derived for determining the conformation of biological macromolecules in solution, adding to the increasing number of shape parameters whose experimental determination does not require a knowledge of the particle swelling due to solvation in solution. lambda can be found from a knowledge of the molecular weight, intrinsic viscosity and the harmonic mean rotational relaxation time. A table of values and a plot of lambda as a function of axial ratio for both oblate and prolate ellipsoids of revolution are given.
摘要
推导出一种新的流体动力学形状函数λ，用于确定溶液中生物大分子的构象，这增加了越来越多的形状参数，其实验测定不需要了解由于在溶液中溶剂化导致的颗粒膨胀情况。可以根据分子量、特性粘度和谐波平均旋转弛豫时间来确定λ。给出了扁长旋转椭球体和长旋转椭球体的λ值表以及λ作为轴比函数的曲线图。
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