Chu H M, Norris D M, Rao K D
Cell Tissue Res. 1980;213(1):1-8. doi: 10.1007/BF00236915.
The prothoracic glands of female pupae of Xyleborus ferrugineus at three ages (0-h, 48-h, and 72-h-old) were examined for ultrastructural changes that correlate with high titers of ecdysteroids in the entire pupa. In all three ages, the prothoracic gland cells appear compact with a prominent nucleus. Lysosome-like structures with concentrically oriented internal membrane are observed in both 0-h and 72-h-old gland cells. Mitochondria are abundant in the glands of both ages, and rough endoplasmic reticulum is relatively sparse. Numerous microtubules are present in the prothoracic gland cells of 48-h-old female pupae that contain relatively high titers of ecdysteroids. Rough endoplasmic reticulum also is especially abundant at this age of the pupa. Ecdysone titers, estimated by the radioimmunoassay method (RIA), were 161.04 +/- 26.80 pg/mg, 704.25 +/- 69.02pg/mg and 298.72 +/- 26.80pg/mg body weight in 0-h, 48-h and 72-h-old female pupae, respectively.
对不同龄期（0小时、48小时和72小时）的锈色长蠹雌性蛹的前胸腺进行了检查，以观察与整个蛹中高滴度蜕皮甾体相关的超微结构变化。在所有这三个龄期，前胸腺细胞看起来紧密，有一个突出的细胞核。在0小时和72小时龄的腺细胞中均观察到具有同心排列内膜的溶酶体样结构。两个龄期的腺体中线粒体都很丰富，粗面内质网相对稀少。在含有相对高滴度蜕皮甾体的48小时龄雌性蛹的前胸腺细胞中有大量微管。在这个蛹龄期，粗面内质网也特别丰富。通过放射免疫测定法（RIA）估计，0小时、48小时和72小时龄雌性蛹的蜕皮酮滴度分别为161.04±26.80 pg/mg、704.25±69.02 pg/mg和298.72±26.80 pg/mg体重。
