Mechanistic aspects of enhanced lipid peroxidation following glutathione depletion in vivo.

Several agents known to conjugate with glutathione (GSH) were administered to phenobarbital-induced rats resulting in a more or less pronounced depletion of hepatic GSH. In vitro incubations showed that a large enhancement of spontaneous lipid peroxidation was observed when the GSH content was below 1 mumol/g liver. This effect was inhibited by addition of exogenous GSH in a concentration-dependent manner, the GSH-concentration yielding 50% inhibition (I50) being 1 microM. Using phorone (diisopropylidene acetone), which proved to be the most potent GSH-depletor, the time- and dose-dependence of the GSH-depletion and the consequent lipid peroxidation was studied. Again it was assured that the GSH concentration must reach a critical value of about 20% of the initial hepatic GSH content, before an enhanced lipid peroxidation is seen. Employing scavengers of excited oxygen species no evidence was found for the involvement of free oxygen radicals. Hepatoprotective agents and inhibitors of mixed-function oxidases exerted a more or less pronounced inhibitory action. Our findings are further support of our previous postulate that GSH depletion per se might lead to an increased lipid peroxidation, possibly due to its lack as a part of the cellular defence system against endogenous toxic intermediates.