紫贻贝（Mytilus edulis L.）的NADP依赖性异柠檬酸脱氢酶。1. 纯化与特性鉴定。

NADP-dependent isocitrate dehydrogenase from the mussel Mytilus edulis L. 1. Purification and characterisation.

作者信息

Head E J

出版信息

Eur J Biochem. 1980 Oct;111(2):575-9. doi: 10.1111/j.1432-1033.1980.tb04973.x.

DOI:10.1111/j.1432-1033.1980.tb04973.x

Abstract

A purification scheme is described for NADP-dependent isocitrate dehydrogenase from the digestive gland of Mytilus edulis. The scheme incorporates three chromatographic steps: hydroxyapatite adsorption, blue-Sepharose affinity chromatography and DEAE-Sephacel ion-exchange chromatography. The subunit molecular weight of the enzyme was 45 000 (+/- 5000) by sodium dodecylsulfate gel electrophoresis. The purified enzyme was homogeneous on dodecylsulfate gels and had a specific activity of 30--50 U/mg protein.

摘要

描述了一种从紫贻贝消化腺中纯化依赖NADP的异柠檬酸脱氢酶的方法。该方法包括三个色谱步骤：羟基磷灰石吸附、蓝色琼脂糖亲和色谱和DEAE-琼脂糖离子交换色谱。通过十二烷基硫酸钠凝胶电泳测定，该酶的亚基分子量为45000（±5000）。纯化后的酶在十二烷基硫酸钠凝胶上呈均一状态，比活性为30-50 U/mg蛋白质。

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