缓冲液诱导的小牛肠碱性磷酸酶激活。
Buffer-induced activation of calf intestinal alkaline phosphatase.
作者信息
Bannister A, Foster R L
出版信息
Eur J Biochem. 1980 Dec;113(1):199-203. doi: 10.1111/j.1432-1033.1980.tb06156.x.
Abstract
The influence of Tris, phosphate ions, imidazole, carbonate ions, bicarbonate ions and hydroxyl ions on the rate of activation of calf intestinal alkaline phosphatase after its dissolution in aqueous solution was investigated. The kinetic data are consistent with a general base-catalysed reaction in which the base reacts with an unprotonated enzyme species formed by ionization of a group with pK = 8.9.
摘要
研究了Tris、磷酸根离子、咪唑、碳酸根离子、碳酸氢根离子和氢氧根离子对小牛肠碱性磷酸酶在水溶液中溶解后活化速率的影响。动力学数据与一般碱催化反应一致，其中碱与由pK = 8.9的基团电离形成的未质子化酶物种反应。
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