Triphaus G F, Schmidt A, Buddecke E
Hoppe Seylers Z Physiol Chem. 1980 Dec;361(12):1773-9. doi: 10.1515/bchm2.1980.361.2.1773.
From human hyaline cartilage (processus xyphoid) preincubated in the presence of [35S]sulfate, proteoglycans were extracted by 4M guanidinium chloride and divided into 6 age groups. Fractionation of proteoglycans by gel filtration under dissociative conditions resulted in two proteoglycan fractions (a and b) with different hydrodynamic volumes. The higher molecular weight fraction a contained chondroitin sulfate, the fraction b keratan sulfate as predominant glycosaminoglycan, the chondroitin sulfate/keratan sulfate ratio decreasing with increasing age in either fraction. The relative portion of proteoglycan fraction b and its 35S-labelling increased with increasing age. From the specific 35S radioactivities of the chondroitin sulfate and keratan sulfate preparations, the occurrence of two independent proteoglycan populations is suggested. A precursor-product relationship between proteoglycan fraction a and b could be excluded.
从在[35S]硫酸盐存在下预孵育的人透明软骨（剑突）中，用4M氯化胍提取蛋白聚糖，并将其分为6个年龄组。在解离条件下通过凝胶过滤对蛋白聚糖进行分级分离，得到两个具有不同流体动力学体积的蛋白聚糖级分（a和b）。分子量较高的级分a含有硫酸软骨素，级分b以硫酸角质素作为主要糖胺聚糖，两个级分中硫酸软骨素/硫酸角质素的比例均随年龄增长而降低。蛋白聚糖级分b的相对比例及其35S标记随年龄增长而增加。根据硫酸软骨素和硫酸角质素制剂的特定35S放射性，提示存在两个独立的蛋白聚糖群体。可以排除蛋白聚糖级分a和b之间的前体-产物关系。
