氯胺 -T对牛生长激素中甲硫氨酸残基的氧化作用。

Oxidation of methionine residues in bovine growth hormone by chloramine-T.

作者信息

Cascone O, Biscoglio de Jimenez Bonino M J, Santomé J A

出版信息

Int J Pept Protein Res. 1980 Oct;16(4):299-305. doi: 10.1111/j.1399-3011.1980.tb02590.x.

DOI:10.1111/j.1399-3011.1980.tb02590.x

PMID:7461909

Abstract

The methionine residues in bovine growth hormone were chemically modified wih chloramine-T at pH 7.4 using different chloramine-T methionine molar ratios. Methionine 4 was shown to be the most reactive followed in decreasing order by methionines 148, 123 and 178. With a 50-fold molar excess of chloramine-T over methionine almost full oxidation of all the methionine residues was achieved. The oxidation of the four residues of methionine does not change the growth promoting activity of the hormone.

摘要

在pH 7.4条件下，使用不同的氯胺-T与甲硫氨酸摩尔比，对牛生长激素中的甲硫氨酸残基进行化学修饰。结果表明，甲硫氨酸4的反应活性最高，其次是甲硫氨酸148、123和178，反应活性依次降低。当氯胺-T的摩尔量比甲硫氨酸过量50倍时，几乎所有甲硫氨酸残基都实现了完全氧化。甲硫氨酸四个残基的氧化不会改变该激素的促生长活性。