Schmidtler W
Histochemistry. 1980;70(1):77-90. doi: 10.1007/BF00508847.
In the ovaries of 75 guinea pigs from 42 days of gestational age to adult animals follicles of every developmental stage were investigated by means of histochemistry for the appearance of acid phosphatase, beta-glucuronidase and non-specific esterase in oocytes and follicle epithelial cells. For morphological control semi-thin sections were examined. Already in primordial follicles, oocytes exhibit a beta-glucuronidase and acid phosphatase reaction. Non-specific esterase can be detected in the oocytes and granulosa cells of secondary follicles. The three of them increase in activity during the growth of the follicles, but show no differences in the ontogenesis of the guinea pig. In the follicle epithelium the reaction of non-specific esterase is especially apparent in the cytoplasm of the cells surrounding the so called "Call-Exner-Bodies". The highest activity is seen in the content of these formations whereas the liquor folliculi does not show a considerable activity. Atresia of follicles can be detected both by enzyme histochemistry and visually in semi-thin sections, at least in advanced stages. The boundaries with intact follicles can at first be recognized by comparison of the follicle epithelium.
对75只从孕龄42天到成年期的豚鼠卵巢进行研究，运用组织化学方法检测处于各个发育阶段卵泡的卵母细胞和卵泡上皮细胞中酸性磷酸酶、β-葡萄糖醛酸酶和非特异性酯酶的表现。为进行形态学对照，对半薄切片进行了检查。在原始卵泡中，卵母细胞就呈现出β-葡萄糖醛酸酶和酸性磷酸酶反应。在次级卵泡的卵母细胞和颗粒细胞中可检测到非特异性酯酶。这三种酶在卵泡生长过程中活性增加，但在豚鼠个体发育过程中无差异。在卵泡上皮中，非特异性酯酶反应在所谓“卡尔-埃克斯纳小体”周围细胞的细胞质中尤为明显。这些结构的内容物中活性最高，而卵泡液中未显示出显著活性。卵泡闭锁在酶组织化学和半薄切片中均可检测到，至少在晚期阶段如此。通过比较卵泡上皮，最初可识别出与完整卵泡的界限。
