Takenaka A, Okamura H, Yajima Y, Okuda Y, Nishimura T
Nihon Sanka Fujinka Gakkai Zasshi. 1980 May;32(5):535-40.
In order to clarify the nature and possible roles of lysosome-like granules observed electronmicroscopically in the granulosa cells of human preovulatory follicles, lysosomal activities in human preovulatory granulosa cells were investigated ultrastructurally by staining a marker enzyme acid phosphatase with modified Gomori technique. In the granulosa cells of follicles 10 approximately 15 mm in diameter, acid phosphatase reaction products were seen only in the Golgi apparatus. In follicles around 20 mm in diameter with extremely thin apical walls, granulosa cells with abundant nexuses and steroidogenic organelles (sER, large spherical mitochondria with tubular cristae and lipid-droplets), had many lysosome-like granules in the perinuclear regions and/or close to lipid droplets. Acid phosphatase reaction products were observed mainly in these granules, and infrequently in Golgi apparatus. These morphological results not only identify such granules as lysosomes, but also indicate that the granulosa cells are rich in the lysosomal activity. Possible roles of lysosomes in preovulatory granulosa cells deduced from the present results are as follows, 1) a role in steroidogenesis or in steroid-secretion, 2) an involvement in the ovulatory process with their proteolytic enzymes.
为了阐明在人排卵前卵泡颗粒细胞中通过电子显微镜观察到的溶酶体样颗粒的性质和可能的作用，采用改良的Gomori技术对标记酶酸性磷酸酶进行染色，通过超微结构研究人排卵前颗粒细胞中的溶酶体活性。在直径约10至15毫米的卵泡颗粒细胞中，酸性磷酸酶反应产物仅见于高尔基体。在直径约20毫米且顶壁极薄的卵泡中，具有丰富连接和类固醇生成细胞器（滑面内质网、带有管状嵴的大球形线粒体和脂滴）的颗粒细胞，在核周区域和/或靠近脂滴处有许多溶酶体样颗粒。酸性磷酸酶反应产物主要在这些颗粒中观察到，在高尔基体中很少见。这些形态学结果不仅将此类颗粒鉴定为溶酶体，还表明颗粒细胞富含溶酶体活性。从目前结果推断，溶酶体在排卵前颗粒细胞中的可能作用如下：1）在类固醇生成或类固醇分泌中的作用；2）通过其蛋白水解酶参与排卵过程。
