Doran D M, Spar I L
J Immunol Methods. 1980;39(1-2):155-63. doi: 10.1016/0022-1759(80)90304-x.
The iodine monochloride (IC1) technique is used to radiolabel proteins under mild experimental conditions. Proteins labeled by this technique have been shown to have both in vitro in vivo characteristics often superior to those proteins labeled by the more frequently used chloramine-T method. Although first published more than two decades ago, use of the IC1 technique has largely been superceded by other more recently developed iodination methods. This paper attempts to reintroduce th oxidative IC1 method to the research community. This is accomplished by the presentation of a detailed protocol for a convenient modification of the original technique. A review of the qualities of the iodinated proteins produced by the IC1 and other iodination methods is also discussed.
一氯化碘（IC1）技术用于在温和的实验条件下对蛋白质进行放射性标记。已证明用该技术标记的蛋白质在体外和体内都具有一些特性，这些特性通常优于用更常用的氯胺-T法标记的蛋白质。尽管一氯化碘技术在二十多年前就首次发表了，但它的应用在很大程度上已被其他最近开发的碘化方法所取代。本文试图将氧化一氯化碘方法重新引入研究领域。这是通过展示一个方便修改原始技术的详细方案来实现的。本文还讨论了对一氯化碘法和其他碘化方法产生的碘化蛋白质特性的综述。
