Gulati A K, Reyer R W
J Exp Zool. 1980 Oct;214(1):109-18. doi: 10.1002/jez.1402140114.
The trophic influence of neural retina in regeneration of the lens from dorsal iris is well known. The first part of this study involved transplantation of dorsal iris pieces into various intra-ocular positions in order to determine the field of action of the neural retina. The most favorable location for lens regeneration was the pupillary space, closely followed by the vitreous chamber. Lens regeneration was retarded from implants lying in the anterior chamber; this was most evident when grafts were placed in front of the iris and not in contact with the swollen vitreous body. Incorporation of 3H-leucine in the neural retina and vitreous body was also investigated using autoradiography. No significant difference in labeling was seen between the control and lentectomized neural retina at all time intervals studied. However, increased labeling above that in the controls was observed over the vitreous body between 8 to 20 days after lentectomy. There was also an increase in the number of silver grains over the vitreous body with increasing time between injection of isotope and sacrifice of the animal. These results provide evidence that vitreous body plays an important role in the process of lens regeneration, perhaps serving as a preferential pathway for transfer of the neural retinal factor to the dorsal iris.
神经视网膜对背侧虹膜晶状体再生的营养作用是众所周知的。本研究的第一部分涉及将背侧虹膜片移植到眼内的不同位置，以确定神经视网膜的作用范围。晶状体再生最有利的位置是瞳孔空间，其次是玻璃体腔。植入物位于前房时，晶状体再生会延迟；当移植物置于虹膜前方且不与肿胀的玻璃体接触时，这种情况最为明显。还使用放射自显影术研究了3H-亮氨酸在神经视网膜和玻璃体中的掺入情况。在所有研究的时间间隔内，对照神经视网膜和晶状体切除后的神经视网膜之间在标记上没有显著差异。然而，在晶状体切除后8至20天之间，玻璃体上观察到的标记高于对照。随着同位素注射与动物处死之间时间的增加，玻璃体上的银粒数量也增加。这些结果证明玻璃体在晶状体再生过程中起重要作用，可能作为神经视网膜因子转移到背侧虹膜的优先途径。
