非钠依赖性γ-氨基丁酸结合对大鼠脑细胞膜制剂中钠依赖性γ-氨基丁酸结合测定的影响。

Influence of sodium-independent gamma-aminobutyric acid binding on the assay of sodium-dependent gamma-aminobutyric acid binding in a membrane preparation of rat brain.

作者信息

Kurioka S, Matsuda M

出版信息

J Neurochem. 1981 Jan;36(1):313-6. doi: 10.1111/j.1471-4159.1981.tb02413.x.

DOI:10.1111/j.1471-4159.1981.tb02413.x

Abstract

A large amount of [3H]GABA was bound to crude synaptic membrane fractions of rat, by sodium-independent process in a medium that contained 100 microM [3H]GABA used for assaying GABA uptake site. This [3H]-GABA binding was different from receptor binding of GABA. It was confirmed that this sodium-independent [3H]GABA binding scarcely occurred in the presence of a physiological concentration of sodium chloride, and that sodium-independent GABA binding had a negligible influence on sodium-dependent GABA binding.

摘要

在用于检测γ-氨基丁酸（GABA）摄取位点的含有100微摩尔[³H]GABA的培养基中，大量的[³H]GABA通过不依赖钠离子的过程与大鼠的粗制突触膜组分结合。这种[³H] - GABA结合不同于GABA的受体结合。已证实，在生理浓度的氯化钠存在下，这种不依赖钠离子的[³H]GABA结合几乎不发生，并且不依赖钠离子的GABA结合对依赖钠离子的GABA结合的影响可忽略不计。