Lindberg I, Dahl J L
J Neurochem. 1981 Feb;36(2):506-12. doi: 10.1111/j.1471-4159.1981.tb01621.x.
Using antisera specific for methionine- and leucine-enkephalin, we studied the characteristics of the release of these peptides from rat striatal slices. Only 2-3% of the total tissue stores of enkephalin could be released by potassium-depolarization; similar percentages were released from globus pallidus, thalamus, and nucleus accumbens. Enkephalin release from hippocampus could not be detected. The striatal release of both enkephalins was affected similarly by changes in potassium and calcium levels in the superfusion medium. Lithium has no effect on either basal or potassium-stimulated release; tyr-arg did not affect basal release of either peptide. Striatal enkephalin levels were stable during the short-term incubation periods used in these experiments.
利用对甲硫氨酸脑啡肽和亮氨酸脑啡肽具有特异性的抗血清，我们研究了这些肽从大鼠纹状体切片中释放的特性。通过钾离子去极化，仅能释放出脑啡肽总组织储存量的2 - 3%；从苍白球、丘脑和伏隔核释放出的比例相似。未检测到海马体中脑啡肽的释放。在灌注培养基中，钾离子和钙离子水平的变化对两种脑啡肽从纹状体的释放产生的影响相似。锂对基础释放或钾离子刺激的释放均无影响；酪氨酰 - 精氨酸对两种肽的基础释放均无影响。在这些实验所采用的短期孵育期间，纹状体脑啡肽水平保持稳定。
