Tsilifonis D C, Chafetz L
J Pharm Sci. 1980 Dec;69(12):1461-3. doi: 10.1002/jps.2600691236.
A specific assay for quinestrol was developed using high-pressure liquid chromatography. The estrogen was separated from tablet excipients on a chemically bonded hydrocarbon column utilizing acetonitrile-water as the mobile phase. Linearity studies were carried out using peak height measurements, and the detector response to the concentration of the steroid was confirmed. This procedure was rapid, accurate precise, and specific for the assay of the synthetic estrogen in the presence of formulation excipients and structurally similar estrogens.
采用高压液相色谱法开发了炔雌醚的特定测定方法。在化学键合烃柱上，以乙腈 - 水为流动相，将雌激素与片剂辅料分离。使用峰高测量进行线性研究，并确认了检测器对甾体浓度的响应。该方法快速、准确、精密，且在存在制剂辅料和结构相似雌激素的情况下，对合成雌激素的测定具有特异性。
