伏杀磷、灭蚜磷及相关化合物的连续薄层色谱法。

Sequential thin-layer chromatography of phosfolan, mephosfolan and related compounds.

作者信息

Bakry N M, Abou-Donia M B

出版信息

J Anal Toxicol. 1980 Jul-Aug;4(4):212-4. doi: 10.1093/jat/4.4.212.

DOI:10.1093/jat/4.4.212

Abstract

A sequential thin-layer chromatographic method (STLC) has been developed to analyze the two organophosphorus insecticides phosfolan (O,O-diethyl 1,3-dithiolan-2-ylidenephosphoramidate) and mephospholan (O,O-diethyl 4-methyl-1,3-dithiolan-2-ylidenephosphoramidate) and some of their possible degradation products. Gelman type SA, Instant Thin Layer Chromatography (ITLC) silicic acid-impregnated glass fiber sheets were first developed up to 6 cm with the primary solvent 2-butanone-1-butanol-water (9:3:1), then after drying, to 16 cm with the secondary solvent acetonitrile-n-hexane-benzene-acetic acid (80:40:40:1). This two-solvent sequential system separated each insecticide from its corresponding metabolites. The R1 values were: phosfolan, 0.69; ethylene dithioimido-carbonate hydrochloride, 0.08; ethylene dithiocarbonate, 0.90; potassium thiocyanate, 0.40; mephosfolan, 0.76; O,O-diethyl 4-hydroxymethyl-1,3-dithiolan-2-amidate, 0.83; propylene dithioimidocarbonate hydrochloride; 0.15 and propylene dithiocarbonate, 0.87.

摘要

已开发出一种顺序薄层色谱法（STLC），用于分析两种有机磷杀虫剂甲拌磷（O,O - 二乙基 - 1,3 - 二硫杂环戊烯 - 2 - 亚基磷酰胺）和灭蚜磷（O,O - 二乙基 - 4 - 甲基 - 1,3 - 二硫杂环戊烯 - 2 - 亚基磷酰胺）及其一些可能的降解产物。首先使用主要溶剂2 - 丁酮 - 1 - 丁醇 - 水（9:3:1）将Gelman SA型即时薄层色谱（ITLC）硅酸浸渍玻璃纤维板展开至6厘米，干燥后，再使用次要溶剂乙腈 - 正己烷 - 苯 - 乙酸（80:40:40:1）展开至16厘米。这种双溶剂顺序系统将每种杀虫剂与其相应的代谢物分离。Rf值分别为：甲拌磷，0.69；乙二硫代亚氨基碳酸盐酸盐，0.08；乙二硫代碳酸酯，0.90；硫氰酸钾，0.40；灭蚜磷，0.76；O,O - 二乙基 - 4 - 羟甲基 - 1,3 - 二硫杂环戊烯 - 2 - 酰胺，0.83；丙二硫代亚氨基碳酸盐酸盐，0.15；丙二硫代碳酸酯，0.87。