Brunner H, Schaar H, Krauss H
Zentralbl Bakteriol A. 1980;248(1):120-8.
Striking alterations, which looked like blebs, were observed in colonies of Acholeplasma laidlawii, strain JA 1. "Craters" were seen on the surface of these colonies by scanning electron microscopy. In addition, giant cells of acholeplasma, up to 14 microns in diameter, i.e. approximately 20 times the size of a normal A. laidlawii cell, were visible in the colonies. The large forms were surrounded by a unit membrane. After infection with group 1 and group 2 mycoplasma-viruses, the proportion of altered colonies and the number of large cells within these colonies increased. A strain of A. laidlawii, which was not susceptible to infection with the three known acholeplasma-viruses did not exhibit comparable morphological changes. The development of the giant cells can be explained either by cell fusion or by a lag of cell division behind genome replication. Blebs and "craters" may result from the destruction of mycoplasma organisms within the complex structure of the colony. There is also suggestive evidence that strain JA 1 carries a virus in some cryptic form.
在莱氏无胆甾原体JA 1菌株的菌落中观察到了明显的变化，这些变化看起来像气泡。通过扫描电子显微镜在这些菌落的表面看到了“火山口”。此外，在菌落中可见直径达14微米的无胆甾原体巨型细胞，即大约是正常莱氏无胆甾原体细胞大小的20倍。这些大型细胞被一层单位膜包围。在用1组和2组支原体病毒感染后，发生变化的菌落比例以及这些菌落内大型细胞的数量增加。一株对三种已知的无胆甾原体病毒感染不敏感的莱氏无胆甾原体菌株未表现出类似的形态变化。巨型细胞的形成可以通过细胞融合或细胞分裂滞后于基因组复制来解释。气泡和“火山口”可能是由于菌落复杂结构内支原体生物体的破坏所致。也有暗示性证据表明JA 1菌株携带某种隐匿形式的病毒。
