Bellet H, Fauve J, Serre A, Castel J, Magnan de Bornier B, Jean R
Arch Fr Pediatr. 1980 May;37(5):345-7.
The difficulties encountered in developing a microassay of plasma aminoacids are presented. Blood was collected by capillary puncture simultaneously at the heel, finger tip and from the lobe of the ear and the results were compared with those obtained from venous blood. Capillary plasma was shown to be contaminated by free aminoacids from the skin, which causes a considerable elevation especially of the serine level. Glycine, ornithine and histidine were also often increased. Our findings suggest that caution is necessary when interpreting aminoacid results estimated on blood collected by capillary puncture.
本文介绍了开发血浆氨基酸微量测定法时遇到的困难。通过足跟、指尖和耳垂的毛细血管穿刺同时采集血液,并将结果与静脉血的结果进行比较。结果表明,毛细血管血浆被来自皮肤的游离氨基酸污染,这导致尤其是丝氨酸水平显著升高。甘氨酸、鸟氨酸和组氨酸也经常增加。我们的研究结果表明,在解释通过毛细血管穿刺采集的血液中氨基酸的测定结果时需要谨慎。