Skriver L, Williams N E
Biochem J. 1980 Jun 15;188(3):695-704. doi: 10.1042/bj1880695.
The synthesis of ciliary-membrane phospholipids and ciliary proteins was studied after deciliation in starving Tetrahymena thermophila cells. Deciliated cells regenerated the new ciliary membrane without any induced phospholipid synthesis. The constant cell volume found during the regrowth of the cilia suggests that renewal of ciliary membranes takes place by insertion of intracellular membrane material into the cell surface. In contrast with the absence of induced phospholipid synthesis during ciliary regeneration, the synthesis of ciliary proteins was found to be induced. This enhanced synthetic activity was made possible by an increased rate of intracellular protein degradation in regenerating cells. It was found that the extent of the induced synthesis strongly depends upon the growth conditions of the cells before starvation. Furthermore, it was shown that the degree of induced protein synthesis is greater for higher-molecular-weight ciliary proteins than for lower-molecular-weight species.
在饥饿的嗜热四膜虫细胞去纤毛后，对纤毛膜磷脂和纤毛蛋白的合成进行了研究。去纤毛细胞在没有任何诱导磷脂合成的情况下再生出新的纤毛膜。纤毛再生过程中细胞体积恒定，这表明纤毛膜的更新是通过将细胞内膜物质插入细胞表面来实现的。与纤毛再生过程中没有诱导磷脂合成相反，发现纤毛蛋白的合成是被诱导的。再生细胞中细胞内蛋白质降解速率的增加使得这种增强的合成活性成为可能。发现诱导合成的程度强烈依赖于饥饿前细胞的生长条件。此外，还表明高分子量纤毛蛋白的诱导蛋白合成程度比低分子量蛋白更高。
