Kishishita M, Ushijima T, Ozaki Y, Ito Y
Appl Environ Microbiol. 1980 Dec;40(6):1100-5. doi: 10.1128/aem.40.6.1100-1105.1980.
The conditions for isolation and cultivation of Propionibacterium acnes and related propionibacteria were studied in detail. Triton X-100 added to the diluent inhibited the growth of propionibacteria in concentrations of 0.05 to 0.1%. However, such was not the case with Tween 80; rather, growth of the bacteria was further enhanced by this agent. Consequently, Tween 80 was considered to be a suitable surfactant for addition to the diluent for isolation of propionibacteria. A new medium for isolating propionibacteria from human skin was developed. Comparative studies with colonies of P. acnes, Propionibacterium granulosum, and Staphylococcus epidermidis showed morphological differences among the colonies; thus, the medium was very useful for differentiating and identifying species of the microbes. The new medium was used for studies on the distribution of propionibacteria on the foreheads of 30 Japanese volunteers. Among 447 strains of P. acnes and 86 strains of P. granulosum isolated from the volunteers, all strains of the former were positive for indole, nitrate, milk, and gelatin hydrolysis, whereas all strains of the latter were negative for all of the tests.
对痤疮丙酸杆菌及相关丙酸杆菌的分离培养条件进行了详细研究。添加到稀释剂中的吐温80在浓度为0.05%至0.1%时会抑制丙酸杆菌的生长。然而，吐温80的情况并非如此；相反，这种试剂会进一步促进细菌的生长。因此，吐温80被认为是一种适合添加到用于分离丙酸杆菌的稀释剂中的表面活性剂。开发了一种从人皮肤中分离丙酸杆菌的新培养基。对痤疮丙酸杆菌、颗粒丙酸杆菌和表皮葡萄球菌菌落的比较研究表明，这些菌落之间存在形态差异；因此，该培养基对于区分和鉴定微生物种类非常有用。这种新培养基被用于研究30名日本志愿者前额上丙酸杆菌的分布情况。从志愿者中分离出的447株痤疮丙酸杆菌和86株颗粒丙酸杆菌中，前者所有菌株对吲哚、硝酸盐、牛奶和明胶水解试验均呈阳性，而后者所有菌株对所有这些试验均呈阴性。
