Karaki H, Weiss G B
Blood Vessels. 1981;18(1-2):28-35. doi: 10.1159/000158335.
Effects of antimycin A, oligomycin and KCN on tension responses to high K+ or NE and on related Ca++ movements were investigated in rabbit aortic smooth muscle. Selected concentrations of antimycin A (10(-6) g/ml), oligomycin (10(-6) g/ml) and KCN (1 mM) had little effect on the K+-induced contractile response even though all three agents abolished the increase in La+++-resistant low affinity Ca++ uptake elicited with high K+. Though the mitochondrial inhibitors had varied effects on the NE-induced contractile response, they had no effect on the NE-induced decrease in La+++-resistant high affinity Ca++. Oxygen consumption of the muscle tissue was decreased by antimycin A or oligomycin. These results suggest that the increase in low affinity Ca++ retention elicited with high K+ represents Ca++ uptake by mitochondria. Thus, the correlation between K+-induced uptake of Ca++ and an increased retention of La+++-resistant low affinity Ca++ after exposure to K+ are sequential events that can be associated by inhibitors of mitochondrial Ca++ uptake.
研究了抗霉素A、寡霉素和氰化钾对兔主动脉平滑肌对高钾或去甲肾上腺素（NE）的张力反应以及相关钙离子运动的影响。抗霉素A（10⁻⁶ g/ml）、寡霉素（10⁻⁶ g/ml）和氰化钾（1 mM）的选定浓度对钾离子诱导的收缩反应影响很小，尽管这三种药物都消除了高钾引起的镧离子抗性低亲和力钙离子摄取的增加。尽管线粒体抑制剂对去甲肾上腺素诱导的收缩反应有不同影响，但它们对去甲肾上腺素诱导的镧离子抗性高亲和力钙离子减少没有影响。抗霉素A或寡霉素可降低肌肉组织的耗氧量。这些结果表明，高钾引起的低亲和力钙离子潴留增加代表线粒体对钙离子的摄取。因此，钾离子诱导的钙离子摄取与暴露于钾离子后镧离子抗性低亲和力钙离子潴留增加之间的相关性是可以被线粒体钙离子摄取抑制剂关联起来的连续事件。
