脑啡肽对大鼠皮层和纹状体切片中乙酰胆碱自发释放和诱发释放作用的研究。
Investigation of action of enkephalin on the spontaneous and evoked release of acetylcholine from rat cortical and striatal slices.
作者信息
Jhamandas K, Elliott J
出版信息
Br J Pharmacol. 1980;71(1):211-7. doi: 10.1111/j.1476-5381.1980.tb10928.x.
1 The effects of two synthetic enkephalins, D-Ala2-Met5-enkephalinamide (DALA) and D-Ala2-MePhe4-Met5-(0)-ol-enkephalin (33,824) were tested on the spontaneous and stimulated release of acetylcholine (ACh) from rat cortical and striatal slices. 2 DALA, but not 33,824, caused a modest increase in spontaneous cortical ACh release. Neither enkephalin affected the release of cortical ACh induced by high potassium (25 mM) or veratridine (20 microM). 3 DALA had no effect on the release of ACh from striatal slices occurring spontaneously or in the presence of potassium (25 mM), veratridine (5 microM) or ouabain (10 microM). The enkephalin 33,824 also had no significant action on the striatal ACh release except that it caused a slight enhancement of veratridine-evoked release. 4 It is suggested that enkephalins have no significant action on release of ACh from the cortex or striatum in vitro. Their action on ACh release from the cortex in vivo, seen in previous studies, may be exerted at a subcortical level.
测试了两种合成脑啡肽，即D - 丙氨酸² - 甲硫氨酸⁵ - 脑啡肽酰胺（DALA）和D - 丙氨酸² - 甲基苯丙氨酸⁴ - 甲硫氨酸⁵ - （O） - 醇 - 脑啡肽（33,824）对大鼠皮质和纹状体切片中乙酰胆碱（ACh）自发释放和刺激释放的影响。
DALA，但不是33,824，导致皮质ACh自发释放适度增加。两种脑啡肽均不影响高钾（25 mM）或藜芦碱（20 μM）诱导的皮质ACh释放。
DALA对纹状体切片中自发或在钾（25 mM）、藜芦碱（5 μM）或哇巴因（10 μM）存在下的ACh释放没有影响。脑啡肽33,824对纹状体ACh释放也没有显著作用，只是它导致藜芦碱诱发的释放略有增强。
提示脑啡肽在体外对皮质或纹状体中ACh的释放没有显著作用。在先前研究中观察到的它们对体内皮质ACh释放的作用可能在皮质下水平发挥。
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