Onagbesan O M, Peddie M J
Department of Physiology and Pharmacology, University of Southampton, UK.
J Reprod Fertil. 1995 Jul;104(2):259-65. doi: 10.1530/jrf.0.1040259.
The effects of insulin-like growth factor I (IGF-I), alone, and in combination with LH or transforming growth factor alpha (TGF-alpha), on replication and progesterone production by cultured avian granulosa cells obtained from the three largest (F1-F3) follicles were studied. IGF-I and TGF-alpha stimulated proliferation of granulosa cells in a dose-dependent manner, and responsiveness decreased as the cells matured. IGF-I stimulated progesterone production from granulosa cells of all the follicles with no change in ED50 value during follicular maturation; however, the maximum response was from cells derived from F1 follicles. IGF-I plus LH had an additive effect on progesterone production by cells from all follicles. In contrast, TGF-alpha inhibited basal and LH- and IGF-I-stimulated progesterone production. These data show that IGF-I and TGF-alpha may interact with each other during granulosa cell maturation, such that efficacy of IGF-I increases, while that of TGF-alpha decreases before ovulation. Furthermore, both growth factors interact with LH, either to enhance or inhibit progesterone production by granulosa cells. However, LH, IGF-I and TGF-alpha combine to stimulate proliferation of granulosa cells.
研究了胰岛素样生长因子I（IGF-I）单独以及与促黄体生成素（LH）或转化生长因子α（TGF-α）联合使用时，对从三个最大卵泡（F1-F3）获得的培养禽颗粒细胞的增殖和孕酮产生的影响。IGF-I和TGF-α以剂量依赖的方式刺激颗粒细胞增殖，并且随着细胞成熟反应性降低。IGF-I刺激所有卵泡的颗粒细胞产生孕酮，在卵泡成熟过程中ED50值无变化；然而，最大反应来自F1卵泡的细胞。IGF-I加LH对所有卵泡细胞的孕酮产生具有相加作用。相比之下，TGF-α抑制基础以及LH和IGF-I刺激的孕酮产生。这些数据表明，IGF-I和TGF-α在颗粒细胞成熟过程中可能相互作用，使得IGF-I的功效增加，而TGF-α的功效在排卵前降低。此外，两种生长因子都与LH相互作用，要么增强要么抑制颗粒细胞的孕酮产生。然而，LH、IGF-I和TGF-α共同刺激颗粒细胞增殖。
