Hordnes K, Digranes A, Haugen I L, Helland D E, Ulstein M, Jonsson R, Haneberg B
Department of Obstetrics and Gynecology, Haukeland University Hospital, Bergen, Norway.
J Reprod Immunol. 1995 Mar;28(3):247-62. doi: 10.1016/0165-0378(95)00925-b.
The cervico-vaginal mucosa is poorly designed for inducing a mucosal immune response, but it can effect such a response evoked at other mucosal sites. This study was undertaken to determine whether colonic-rectal immunization with group B streptococci (GBS) might induce a local cervico-vaginal immune response. Mice were immunized with either fragmented GBS rectally, whole GBS rectally, or whole GBS subcutaneously. Cholera toxin (CT) was used as an adjuvant for the rectal immunizations. Following colonic-rectal immunization with whole GBS, the mean anti-GBS IgA antibody level in vaginal secretions was 735 kU/ml, with individual values reaching 3480 kU/ml. Corresponding levels of IgA antibodies never exceeded 10 kU/ml in serum and intestinal secretions, or 90 kU/g in feces. In vaginal secretions IgA antibodies to GBS also constituted a much larger fraction of total IgA than in serum, intestinal secretions and feces. Immunizations with fragmented GBS produced much lower IgA responses. Anti-GBS IgA response at the inductive site in the colon-rectum was not significant, as opposed to a strong anti-CT IgA response. Except in serum, the anti-GBS IgG responses to colonic-rectal immunizations were generally low, or absent. The results may provide a basis for the development of mucosal vaccines against GBS-infection.
宫颈阴道黏膜不利于诱导黏膜免疫反应，但它能影响在其他黏膜部位引发的此类反应。本研究旨在确定用B族链球菌（GBS）进行结肠直肠免疫是否可能诱导局部宫颈阴道免疫反应。将小鼠分别用GBS片段进行直肠免疫、用完整GBS进行直肠免疫或用完整GBS进行皮下免疫。霍乱毒素（CT）用作直肠免疫的佐剂。在用完整GBS进行结肠直肠免疫后，阴道分泌物中的平均抗GBS IgA抗体水平为735 kU/ml，个别值达到3480 kU/ml。血清、肠分泌物中的IgA抗体相应水平从未超过10 kU/ml，粪便中的相应水平从未超过90 kU/g。在阴道分泌物中，抗GBS IgA抗体在总IgA中所占比例也比血清、肠分泌物和粪便中的大得多。用GBS片段进行免疫产生的IgA反应要低得多。与强烈的抗CT IgA反应相反，结肠直肠诱导部位的抗GBS IgA反应不显著。除血清外，结肠直肠免疫后的抗GBS IgG反应通常较低或不存在。这些结果可能为开发针对GBS感染的黏膜疫苗提供依据。
