Itoh F, Yamaoka K, Ogino T, Kanbe M
Hiroshima City Institute of Public Health.
Rinsho Byori. 1995 Aug;43(8):772-5.
To identify diarrhea--Escherichia coli(E. coli), especially Enteroinvasive E. coli(EIEC), Verocytotoxin-producing E. coli(VTEC), and Enterotoxigenic E. coli(ETEC), confirmation of pathogenic factors is necessary. We developed a system using the polymerase chain reaction(PCR) that simultaneously confirms the pathogenic genes of these E. coli, and have used it in routine examination. This method is simple and does not require examination for each pathogenic factor. Multiple pathogenic genes could be rapidly detected.
为了鉴定腹泻型大肠杆菌(E. coli),尤其是侵袭性大肠杆菌(EIEC)、产志贺毒素大肠杆菌(VTEC)和产肠毒素大肠杆菌(ETEC),确认致病因素是必要的。我们开发了一种利用聚合酶链反应(PCR)的系统,该系统可同时确认这些大肠杆菌的致病基因,并已将其用于常规检测。这种方法简单,无需对每个致病因素进行检测。多种致病基因能够被快速检测出来。