Toyota K, Nagamori S, Kashiwagi A, Nonomura K, Koyanagi T
Department of Urology, Hokkaido University School of Medicine, Sapporo, Japan.
Nihon Hinyokika Gakkai Zasshi. 1995 Aug;86(8):1353-9. doi: 10.5980/jpnjurol1989.86.1353.
To evaluate the clinical relevance between the DNA ploidy and histopathology, and the incidence of the DNA heterogeneity in patients with bladder cancers.
Flow cytometry (FCM) was used to study the DNA ploidy in 63 patients who underwent total cystectomy. The DNA ploidy and DNA index were analyzed by FCM in total 328 paraffin embedded samples (5.2 samples per case on the average).
The DNA ploidy of 52 bladder cancers, that had coexisted after total cystectomy, showed that 24 cases, 46% were DNA aneuploid and 18 cases, 35% had DNA heterogeneity. The DNA ploidy of 11 cases that were eradicated after cystectomy was all DNA diploid. There were significantly good correlation among DNA ploidy pattern and intravesical involvement (lymph duct involvement and venous involvement), but were not among the DNA ploidy pattern and tumor grade and stage. With regard to the evaluation of two vertical divided samples of tumors, DNA aneuploid had been not always recognized in the deeper sample, therefore, we did not determine that there was good correlation between the DNA ploidy and the tumor invasion.
These data suggest that although the incidence of DNA heterogeneity in bladder cancers (35%) is thought to be relatively small, the DNA ploidy will be able to the important prognosticating factor in bladder cancers.
评估膀胱癌患者DNA倍体与组织病理学之间的临床相关性以及DNA异质性的发生率。
采用流式细胞术（FCM）研究63例行全膀胱切除术患者的DNA倍体。对总共328个石蜡包埋样本（平均每例5.2个样本）进行FCM分析DNA倍体和DNA指数。
全膀胱切除术后共存的52例膀胱癌的DNA倍体显示，24例（46%）为DNA非整倍体，18例（35%）存在DNA异质性。膀胱切除术后根治的11例病例的DNA倍体均为DNA二倍体。DNA倍体模式与膀胱内侵犯（淋巴管侵犯和静脉侵犯）之间存在显著的良好相关性，但与肿瘤分级和分期之间不存在相关性。关于肿瘤的两个垂直分割样本的评估，在较深的样本中并不总是能识别出DNA非整倍体，因此，我们并未确定DNA倍体与肿瘤浸润之间存在良好的相关性。
这些数据表明，尽管膀胱癌中DNA异质性的发生率（35%）被认为相对较小，但DNA倍体将能够成为膀胱癌重要的预后因素。
