Beckett S, Marsden C A
Department of Physiology and Pharmacology, University Medical School Nottingham, Queens Medical Centre, UK.
J Neurosci Methods. 1995 May;58(1-2):157-61. doi: 10.1016/0165-0270(94)00170-l.
The pharmacological study of midbrain-evoked aversive behaviours is commonly measured in terms of electrical escape thresholds, although studies which examine the actual expression of the behaviour are being introduced. A computer-driven automated tracking system has been developed to record and analyse the very rapid locomotor activity produced by the defence response. Stimulation of the midbrain periaqueductal grey (PAG) matter in rats with the excitatory amino acid D,L-homocysteic acid (DLH) produced very rapid and short-lasting escape behaviour which was tracked by computer. The system, VideoTrack, determines the speed and distance travelled by the animal and stores and statistically analyses the data. VideoTrack was able to detect a pharmacological modifications of the response; anti-aversive effects produced by intra-PAG pretreatment with the 5-HT1A agonist 8-hydroxy 2-(di-n-propylamino)tetralin (8-OHDPAT) and its reversal by peripheral pretreatment with the 5-HT1A antagonist N-[2-[-(2-methoxyphenyl)-1-piperazinyl]ethyl-N- (pyridinyl)cyclohexanecarboxanide (WAY 100635).
中脑诱发厌恶行为的药理学研究通常根据电逃避阈值来衡量，尽管目前也有一些研究开始关注该行为的实际表现。我们开发了一种计算机驱动的自动跟踪系统，用于记录和分析防御反应所产生的非常快速的运动活动。用兴奋性氨基酸D，L-高半胱氨酸（DLH）刺激大鼠中脑导水管周围灰质（PAG）会产生非常快速且持续时间短的逃避行为，该行为由计算机进行跟踪。VideoTrack系统可以测定动物的速度和行进距离，并存储数据并进行统计分析。VideoTrack能够检测到反应的药理学改变；PAG内预先用5-HT1A激动剂8-羟基-2-（二正丙基氨基）四氢化萘（8-OHDPAT）预处理会产生抗厌恶作用，而外周预先用5-HT1A拮抗剂N-[2-[（2-甲氧基苯基）-1-哌嗪基]乙基-N-（吡啶基）环己烷甲酰胺（WAY 100635）预处理会逆转这种作用。
