Dong W F, Xu Y, Hu Q L, Munroe D, Minowada J, Housman D E, Minden M D
Ontario Cancer Institute, Princess Margaret Hospital, Department of Medicine, Canada.
Leukemia. 1995 Nov;9(11):1812-7.
Recurrent chromosome translocations involving 11p13 and 14q11 are found in 5-10% of cases of T-ALL. The gene involved in the translocation on chromosome 14 is the T cell antigen receptor alpha or delta. The putative oncogene on chromosome 11 is rhombotin 2 (RBTN2)/translocated in T cell gene 2 (ttg-2), a member of the LIM family of proteins. In this paper we characterize a cell line KOPT-K1 that has a t(11;14)(p13;q11). The breakpoint on chromosome 11 involves an Alu-rich region with the break occurring between two Alu sequences on chromosome 11. In addition, approximately 70 bases from the break on chromosome 11 is a tetranucleotide repeat. Whether either of these structures played a role in the translocation is not known. No heptamer or nonamer sequences, implicated in other rearrangements were found near the breakpoint. The breakpoint on chromosome 11 maps more centromeric than previous translocations of this region. Despite this the RBTN2 gene is highly expressed in KOPT-K1. This cell line will be useful for investigating the role of RBTN2 in leukemogenesis and the mechanism by which the translocation alters the expression of RBTN2.
在5%-10%的T细胞急性淋巴细胞白血病（T-ALL）病例中发现了涉及11p13和14q11的复发性染色体易位。14号染色体上参与易位的基因是T细胞抗原受体α或δ。11号染色体上的推定癌基因是菱蛋白2（RBTN2）/T细胞基因2易位基因（ttg-2），它是LIM蛋白家族的成员。在本文中，我们鉴定了一株具有t(11;14)(p13;q11)的细胞系KOPT-K1。11号染色体上的断点涉及一个富含Alu的区域，断点发生在11号染色体上的两个Alu序列之间。此外，11号染色体断点处约70个碱基处是一个四核苷酸重复序列。这些结构是否在易位中起作用尚不清楚。在断点附近未发现与其他重排有关的七聚体或九聚体序列。11号染色体上的断点比该区域以前的易位更靠近着丝粒。尽管如此，RBTN2基因在KOPT-K1中高度表达。该细胞系将有助于研究RBTN2在白血病发生中的作用以及易位改变RBTN2表达的机制。
